SPF級1-10日齡乳鼠，ICR、C57或Balb/c均可。

設(shè)計方案如下：

Pax1 paired box 1 [ Mus musculus (house mouse) ]

Gene ID: 18503

Also known as: un; wt; hbs; Pax-1; hunchback; undulated

Location: 2 G2; 2 72.63 cM

Exon count: 6

Chromosome 2 - NC_000068.7

Homo: exon2:c.C638G:p.S213W

Mus: exon2:c.CT611-612GG:p.S204W

m-PAX1-gRNA up1      5’TAGGACTGATGGAGCTCACAGA

M-PAX1-gRNA down1    5’aaactctgtgagctccatcagt

Objective

5’accggctactggctgatggcgtttgtgacaagtacaacgtgccctctgtgagctccatcagtcgcatcctgcgaaataagattggcagcc

mut

5’accggctactggctgatggcgtttgtgacaagtacaacgtgccctGGgtgagctccatcagtcgcatcctgcgaaataagattggcagcc

m-PAX1-oligo

5’GGCTGCCAATCTTATTTCGCAGGATGCGACTGATGGAGCTCACCCAGGGCACGTTGTACTTGTCACAAACGCCATCAGCCAGTAGCCGGT